The US President sent a clear warning against Iran as tensions in the Middle East flared up. He made the remarks during a meeting with Israeli President Reuven Rivlin in the White House.
The 46th President said: “What I can say to you Iran will never get a nuclear weapon on my watch as they say.”
He also told the Israeli president the US had an “ironclad” responsibility toward Israel’s defence.
Mr Biden added: “We’re committed to – unwavering commitment to your self defence.
“It's real. It's something that I often say: If there weren't an Israel we'd have to invent one.”
On Sunday, Mr Biden ordered a round of airstrikes against Iran-backed militia in response to strikes by the militia on US facilities in Iraq.
The US military addressed the airstrikes in a statement.
It confirmed that “operational and weapons storage facilities" at two locations in Syria and one location in Iraq were targeted.”
It is the second time Mr Biden has responded to Iran-backed militia since taking office at the beginning of the year.
In February, strikes were ordered on a location in Syria in retaliation to rocket attacks from Iraq.