Olivia Harrison, 13, claims she was ordered to change into clothes from the lost property by a staff member at John Smeaton Academy, Leeds, during lunchtime on Monday. The year nine pupil refused to wear lost property trousers and was sent to isolation.
After objecting to comply with this punishment, she was excluded for two days and handed a further three days in isolation upon her return to school.
Miss Harrison told LeedsLive: "[The staff member] said I had to change my trousers and socks because you could see my ankles and she said it was distracting for other pupils and teachers.
"I said, 'are you saying they are going to get turned on?' And [the staff member] said, 'well I wouldn't put it like that.' She said my trousers showed my figure too much."
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Mum, Karen Harrison, said: “Olivia's trousers are from Debenhams, they are the ones she has wore before with no problems. Now I have got to order more trousers.
"They should be more interested in education and not what pupils are wearing."
Ms Harrison claims the school’s uniform rules have become stricter since it was taken over by The Gorse Academies Trust at the start of this school year.
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A spokesperson for the trust said: “We have the highest standards across all areas of school life, whether that is in the classroom, in terms of behaviour and with regard to uniform.
"We make no apologies for this and are pleased that the majority of students abide by these standards. However, where students do not meet them, we have clear policies in place.
"We do not comment on individual cases but can say we have not received a parental complaint on this matter."
Additional reporting by Ben Abiss.